Correlation of fluorescence microscopy, electron microscopy, and NanoSIMS stable isotope imaging on a single tissue section.

ABSTRACT: Correlative light and electron microscopy allows localization of specific molecules at the ultrastructural level in biological tissue but does not provide information about metabolic turnover or the distribution of labile molecules, such as micronutrients. We present a method to directly correlate (immuno)fluorescent microscopy, (immuno)TEM imaging and NanoSIMS isotopic mapping of the same tissue section, with nanometer-scale spatial precision. The process involves chemical fixation of the tissue, cryo sectioning, thawing, and air-drying under a thin film of polyvinyl alcohol. It permits to effectively retain labile compounds and strongly increases NanoSIMS sensitivity for ¹³C-enrichment. The method is illustrated here with correlated distribution maps of a carbonic anhydrase en

SUBMITTER: Loussert-Fonta C 

PROVIDER: S-EPMC7347930 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature