Cloning and characterization of the rat Slo3 (K_Ca 5.1) channel: From biophysics to pharmacology.

ABSTRACT:

Background and purpose

The Slo3 potassium (K_Ca 5.1) channel, which is specifically expressed in the testis and sperm, is essential for mammalian male fertilization. The sequence divergence of the bovine, mouse and human Slo3 α-subunit revealed a rapid evolution rate across different species. The rat Slo3 (rSlo3) channel has not been cloned and characterized previously.

Experimental approach

We used molecular cloning, electrophysiology (inside-out patches and outside-out patches) and mutagenesis to investigate the biophysical properties and pharmacological characteristics of the rSlo3 channel.

Key results

The rat Slo3 channel (rSlo3) is gated by voltage and cytosolic pH rather than intracellular calcium. The characteristics of voltage-dependent, pH-sensitivi

SUBMITTER: Wang GM 

PROVIDER: S-EPMC7348093 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature