Asiaticoside might attenuate bleomycin-induced pulmonary fibrosis by activating cAMP and Rap1 signalling pathway assisted by A2AR.

ABSTRACT: Asiaticoside (AS) has been reported to have protective effect on pulmonary fibrosis (PF). In this study, we aimed to explore the potential mechanism of the therapeutic role of AS and its relationship with A2AR in PF. Adenosine 2A receptor gene knockout (A2AR^-/- ) mice and wild-type (WT) mice were used to establish bleomycin (BLM)-induced PF models and were then treated with AS (50 mg/kg/d). Pulmonary inflammation and fibrosis were observed in the PF model with much higher severity in A2AR^-/- mice than that in WT mice and AS significantly alleviated lung inflammation and fibrosis; however, it was less effective in A2AR^-/- mice than in WT mice via histopathological analysis. Using RNA sequencing analysis, we found up-regulated differentially expressed genes (

SUBMITTER: Luo J 

PROVIDER: S-EPMC7348182 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature