Modulation of Response Regulator CheY Reaction Kinetics by Two Variable Residues That Affect Conformation.

ABSTRACT: Microorganisms and plants utilize two-component systems to regulate adaptive responses to changing environmental conditions. Sensor kinases detect stimuli and alter their autophosphorylation activity accordingly. Signal propagation occurs via the transfer of phosphoryl groups from upstream kinases to downstream response regulator proteins. Removal of phosphoryl groups from the response regulator typically resets the system. Members of the same protein family may catalyze phosphorylation and dephosphorylation reactions with different efficiencies, exhibiting rate constants spanning many orders of magnitude to accommodate response time scales from milliseconds to days. We previously found that variable positions one or two residues to the C-terminal side of the conserved Asp phosphorylation

SUBMITTER: Straughn PB 

PROVIDER: S-EPMC7348551 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature