Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells.

ABSTRACT: The lack of robust methods to preserve, purify and in vitro maintain the phenotype of the human liver's highly specialized parenchymal and non-parenchymal cell types importantly hampers their exploitation for the development of research and clinical applications. There is in this regard a growing interest in the use of tissue-specific extracellular matrix (ECM) to provide cells with an in vitro environment that more closely resembles that of the native tissue. In the present study, we have developed a method that allows for the isolation and downstream application of the human liver's main cell types from cryopreserved material. We also isolated and solubilized human liver ECM (HL-ECM), analyzed its peptidomic and proteomic composition by mass spectrometry and evaluated its interest for th

SUBMITTER: Alevra Sarika N 

PROVIDER: S-EPMC7349413 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature