The Function of Mitochondrial Calcium Uniporter at the Whole-Cell and Single Mitochondrion Levels in WT, MICU1 KO, and MICU2 KO Cells.

ABSTRACT: Mitochondrial Ca²⁺ ([Ca²⁺]_M) uptake through its Ca²⁺ uniporter (MCU) is central to many cell functions such as bioenergetics, spatiotemporal organization of Ca²⁺ signals, and apoptosis. MCU activity is regulated by several intrinsic proteins including MICU1, MICU2, and EMRE. While significant details about the role of MICU1, MICU2, and EMRE in MCU function have emerged recently, a key challenge for the future experiments is to investigate how these regulatory proteins modulate mitochondrial Ca²⁺ influx through MCU in intact cells under pathophysiological conditions. This is further complicated by the fact that several variables affecting MCU function change dynamically as cell functions. To overcome this void, we develop a d

SUBMITTER: Shah SI 

PROVIDER: S-EPMC7349584 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature