Cell Type-Specific In Vitro Gene Expression Profiling of Stem Cell-Derived Neural Models.

ABSTRACT: Genetic and genomic studies of brain disease increasingly demonstrate disease-associated interactions between the cell types of the brain. Increasingly complex and more physiologically relevant human-induced pluripotent stem cell (hiPSC)-based models better explore the molecular mechanisms underlying disease but also challenge our ability to resolve cell type-specific perturbations. Here, we report an extension of the RiboTag system, first developed to achieve cell type-restricted expression of epitope-tagged ribosomal protein (RPL22) in mouse tissue, to a variety of in vitro applications, including immortalized cell lines, primary mouse astrocytes, and hiPSC-derived neurons. RiboTag expression enables depletion of up to 87 percent of off-target RNA in mixed species co-cultures. Nonetheles

SUBMITTER: Gregory JA 

PROVIDER: S-EPMC7349756 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature