Insights Into Potato Spindle Tuber Viroid Quasi-Species From Infection to Disease.

ABSTRACT: Viroids are non-coding RNA plant pathogens that are characterized by their possession of a high mutation level. Although the sequence heterogeneity in viroid infected plants is well understood, shifts in viroid population dynamics due to mutations over the course of infection remain poorly understood. In this study, the ten most abundant sequence variants of potato spindle tuber viroid RG1 (PSTVd) expressed at different time intervals in PSTVd infected tomato plants were identified by high-throughput sequencing. The sequence variants, forming a quasi-species, were subjected to both the identification of the regions favoring mutations and the effect of the mutations on viroid secondary structure and viroid derived small RNAs (vd-sRNA). At week 1 of PSTVd infection, 25% of the sequence varia

SUBMITTER: Adkar-Purushothama CR 

PROVIDER: S-EPMC7349936 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature