Proximity Ligation Assay as a Tool for Antibody Validation in Human Tissues.

ABSTRACT: Immunohistochemistry (IHC) is the accepted standard for spatial analysis of protein expression in tissues. IHC is widely used for cancer diagnostics and in basic research. The development of new antibodies to proteins with unknown expression patterns has created a demand for thorough validation. We have applied resources from the Human Protein Atlas project and the Antibody Portal at National Cancer Institute to generate protein expression data for 12 proteins across 39 cancer cell lines and 37 normal human tissue types. The outcome of IHC on consecutive sections from both cell and tissue microarrays using two independent antibodies for each protein was compared with in situ proximity ligation (isPLA), where binding by both antibodies is required to generate detection signals. Semi-quantit

SUBMITTER: Lindskog C 

PROVIDER: S-EPMC7350078 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature