ELISA-Based Assay for Studying Major and Minor Group Rhinovirus-Receptor Interactions.

ABSTRACT: Rhinovirus (RV) infections are a major cause of recurrent common colds and trigger severe exacerbations of chronic respiratory diseases. Major challenges for the development of vaccines for RV include the virus occurring in the form of approximately 160 different serotypes, using different receptors, and the need for preclinical models for the screening of vaccine candidates and antiviral compounds. We report the establishment and characterization of an ELISA-based assay for studying major and minor group RV-receptor interactions. This assay is based on the interaction of purified virus with plate-bound human receptor proteins, intercellular adhesion molecule 1 (ICAM-1), and low density lipoprotein receptor (LDLR). Using RV strain-specific antibodies, we demonstrate the specific binding of

SUBMITTER: Pazderova P 

PROVIDER: S-EPMC7350259 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature