Identification of Immunogenic Antigens of Naegleria fowleri Adjuvanted by Cholera Toxin.

ABSTRACT: The intranasal administration of Naegleria fowleri lysates plus cholera toxin (CT) increases protection against N. fowleri meningoencephalitis in mice, suggesting that humoral immune response mediated by antibodies is crucial to induce protection against the infection. In the present study, we applied a protein analysis to detect and identify immunogenic antigens from N. fowleri, which might be responsible for such protection. A Western blot assay of N. fowleri polypeptides was performed using the serum and nasal washes from mice immunized with N. fowleri lysates, either alone or with CT after one, two, three, or four weekly immunizations and challenged with trophozoites of N. fowleri. Immunized mice with N. fowleri plus CT, after four doses

SUBMITTER: Rojas-Hernandez S 

PROVIDER: S-EPMC7350353 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature