Cryptochrome 1 Alleviates the Antiproliferative Effect of Isoproterenol on Human Gastric Cancer Cells.

ABSTRACT:

Background

Cryptochrome 1 (CRY1) is a key protein that regulates the feedback loop of circadian clock. The abnormal expression of CRY1 was reported in numerous cancers, and contributed to tumorigenesis and progression. But the underlying mechanism remains undefined.

Methods

CRY1 overexpression was constructed by lentivirus vector. Gene and protein expression was detected by reverse transcription quantitative polymerase chain reaction and Western blot. Cell proliferation was analyzed by CCK-8 assay. Cell migration ability was analyzed by scratch assay and transwell migration assay. The cAMP concentration was measured by intracellular cAMP assay.

Results

Overexpression of CRY1 showed slightly effect on the proliferation and migration of HGC-27 cells. Upon

SUBMITTER: Huang Q 

PROVIDER: S-EPMC7350398 | biostudies-literature | 2020 Jul-Sep

REPOSITORIES: biostudies-literature