Activation of OX40 and CD27 Costimulatory Signalling in Sheep through Recombinant Ovine Ligands.

ABSTRACT: Members of the tumour necrosis factor (TNF) superfamily OX40L and CD70 and their receptors are costimulating signalling axes critical for adequate T cell activation in humans and mice but characterisation of these molecules in other species including ruminants is lacking. Here we cloned and expressed the predicted ovine orthologues of the receptors OX40 and CD27, as well as soluble recombinant forms of their potential ovine ligands, OaOX40L and OaCD70. Using biochemical and immunofluorescence analyses, we show that both signalling axes are functional in sheep. We show that oligomeric recombinant ligand constructs are able to induce signalling through their receptors on transfected cells. Recombinant defective human adenoviruses were constructed to express the soluble forms of

SUBMITTER: Rojas JM 

PROVIDER: S-EPMC7350415 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature