Activity of fungal β-glucosidases on cellulose.

ABSTRACT:

Background

Fungal beta-glucosidases (BGs) from glucoside hydrolase family 3 (GH3) are industrially important enzymes, which convert cellooligosaccharides into glucose; the end product of the cellulolytic process. They are highly active against the β-1,4 glycosidic bond in soluble substrates but typically reported to be inactive against insoluble cellulose.

Results

We studied the activity of four fungal GH3 BGs on cellulose and found significant activity. At low temperatures (10 ℃), we derived the approximate kinetic parameters k _cat = 0.3 ± 0.1 s^-1 and K _M  = 80 ± 30 g/l for a BG from Aspergillus fumigatus (AfBG) acting on Avicel. Interestingly, this maximal turnover is higher than reported values for typica

SUBMITTER: Keller MB 

PROVIDER: S-EPMC7350674 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature