M⁶A modification-mediated BATF2 acts as a tumor suppressor in gastric cancer through inhibition of ERK signaling.

ABSTRACT:

Background

BATF2, also known as SARI, has been implicated in tumor progression. However, its role, underlying mechanisms, and prognostic significance in human gastric cancer (GC) are elusive.

Methods

We obtained GC tissues and corresponding normal tissues from 8 patients and identified BATF2 as a downregulated gene via RNA-seq. qRT-PCR and western blotting were applied to examine BATF2 levels in normal and GC tissues. The prognostic value of BATF2 was elucidated using tissue microarray and IHC analyses in two independent GC cohorts. The functional roles and mechanistic insights of BATF2 in GC growth and metastasis were evaluated in vitro and in vivo.

Results

BATF2 expression was significantly decreased in GC tissues at both the mRNA and protein level. Multivariate Cox

SUBMITTER: Xie JW 

PROVIDER: S-EPMC7350710 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature