One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies.

ABSTRACT:

Background

Spider silk is a proteinaceous fiber with remarkable mechanical properties spun from spider silk proteins (spidroins). Engineering spidroins have been successfully produced in a variety of heterologous hosts and the most widely used expression system is Escherichia coli (E. coli). So far, recombinantly expressed spidroins often form insoluble inclusion bodies (IBs), which will often be dissolved under extremely harsh conditions in a traditional manner, e.g. either 8 mol/L urea or 6 mol/L guanidine hydrochloride, highly risking to poor recovery of bioactive proteins as well as unexpected precipitations during dialysis process.

Results

Here, we present a mild solubilization strategy-one-step heating method to solubilize spidroins from IBs, with combining spidroins'

SUBMITTER: Cai H 

PROVIDER: S-EPMC7350728 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature