MKL-1 is a coactivator for STAT5b, the regulator of Treg cell development and function.

ABSTRACT:

Background

Foxp3⁺CD4⁺ regulatory T cells (Treg) constitutes a key event in autoimmune diseases. STAT5b is the critical link between the IL-2/15 and FOXP3, the master regulator of Treg cells.

Methods

The CD3⁺T cell and Foxp3⁺CD4⁺ regulatory T cells were overexpressioned or knockdown MKL-1 and STAT5a and tested for Treg cell development and function. Direct interaction of MKL-1 and STAT5a were analyzed by coimmunoprecipitation assays, Luciferase assay, Immunofluoresence Staining and Yeast two-hybrid screening. The effect of MKL-1 and STAT5a on the Treg genes expression was analyzed by qPCR and western blotting and Flow cytometry.

Results

However, the molecular mechanisms mediating STAT5b-dependent Treg genes express

SUBMITTER: Xiang Y 

PROVIDER: S-EPMC7350762 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature