Effect of blood flow on platelets, leukocytes, and extracellular vesicles in thrombosis of simulated neonatal extracorporeal circulation.

ABSTRACT:

Background

Extracorporeal membrane oxygenation (ECMO) has frequent and sometimes lethal thrombotic complications. The role that activated platelets, leukocytes, and small (0.3-micron to 1-micron) extracellular vesicles (EVs) play in ECMO thrombosis is not well understood.

Objectives

To test the effect of blood flow rate on the generation of activated platelets, leukocytes, and EVs in a simulated neonatal ECMO circuit using heparinized human whole blood.

Methods

Simulated neonatal roller pump circuits circulated whole blood at low, nominal, and high flow rates (0.3, 0.5, and 0.7 L/min) for 6 h. Coagulopathy was defined by thromboelastography (TEG), STA^® -procoagulant phospholipid clot time (STA^®- Procoag-PPL), and calibrated automated thrombogram.

SUBMITTER: Meyer AD 

PROVIDER: S-EPMC7350929 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature