Endogenous miR-204 Protects the Kidney against Chronic Injury in Hypertension and Diabetes.

ABSTRACT:

Background

Aberrant microRNA (miRNA) expression affects biologic processes and downstream genes that are crucial to CKD initiation or progression. The miRNA miR-204-5p is highly expressed in the kidney but whether miR-204-5p plays any role in the development of chronic renal injury is unknown.

Methods

We used real-time PCR to determine levels of miR-204 in human kidney biopsies and animal models. We generated Mir204 knockout mice and used locked nucleic acid-modified anti-miR to knock down miR-204-5p in mice and rats. We used a number of physiologic, histologic, and molecular techniques to analyze the potential role of miR-204-5p in three models of renal injury.

Results

Kidneys of patients with hypertension, hypertensive nephrosclerosis, or diabetic nephropathy

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC7350998 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature