Characterization of post-edited cells modified in the TFAM gene by CRISPR/Cas9 technology in the bovine model.

ABSTRACT: Gene editing in large animal models for future applications in translational medicine and food production must be deeply investigated for an increase of knowledge. The mitochondrial transcription factor A (TFAM) is a member of the HMGB subfamily that binds to mtDNA promoters. This gene maintains mtDNA, and it is essential for the initiation of mtDNA transcription. Lately, we generated a new cell line through the disruption of the TFAM gene in bovine fibroblast cells by CRISPR/Cas 9 technology. We showed that the CRISPR/Cas9 design was efficient through the generation of heterozygous mutant clones. In this context, once this gene regulates the mtDNA replication specificity, the study aimed to determine if the post-edited cells are capable of in vitro maintenance and assess if they present c

SUBMITTER: de Oliveira VC 

PROVIDER: S-EPMC7351154 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature