Enhanced wound healing via collagen-turnover-driven transfer of PDGF-BB gene in a murine wound model.

ABSTRACT: Wound healing is a complex biological process that requires coordinated cell proliferation, migration, and extracellular matrix production/remodeling, all of which are inhibited/delayed in chronic wounds. In this study, a formulation was developed that marries a fibrin-based, provisional-like matrix with collagen mimetic peptide (CMP)/PDGF gene-modified collagens, leading to the formation of robust gels that supported temporally controlled PDGF expression and facile application within the wound bed. Analysis employing in vitro co-gel scaffolds confirmed sustained and temporally controlled gene release based on matrix metalloproteinase (MMP) activity, with ~30% higher PDGF expression in MMP producing fibroblasts as-compared with non-MMP-expressing cells. The integration of fibrin with the g

SUBMITTER: Thapa RK 

PROVIDER: S-EPMC7351314 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature