VIPER^nano: Improved Live Cell Intracellular Protein Tracking.

ABSTRACT: Tracking intracellular proteins in live cells has many challenges. The most widely used method, fluorescent protein fusions, can track proteins in their native cellular environment and has led to significant discoveries in cell biology. Fusion proteins add steric bulk to the target protein and can negatively affect native protein function. The use of exogenous probes such as antibodies or protein labels is problematic because these cannot cross the plasma membrane on their own and thus cannot label intracellular targets in cells. We developed a labeling platform, VIPER^nano, for live cell imaging of intracellular proteins using a peptide fusion tag (CoilE) to the protein of interest and delivery of a fluorescently labeled probe peptide (CoilR). CoilR and CoilE form an α-helical h

SUBMITTER: Morgan E 

PROVIDER: S-EPMC7351371 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature