Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes.

ABSTRACT: Traditional cultivation approaches in microbiology are labor-intensive, low-throughput, and yield biased sampling of environmental microbes due to ecological and evolutionary factors. New strategies are needed for ample representation of rare taxa and slow-growers that are often outcompeted by fast-growers in cultivation experiments. Here we describe a microfluidic platform that anaerobically isolates and cultivates microbial cells in millions of picoliter droplets and automatically sorts them based on colony density to enhance slow-growing organisms. We applied our strategy to a fecal microbiota transplant (FMT) donor stool using multiple growth media, and found significant increase in taxonomic richness and larger representation of rare and clinically relevant taxa among droplet-grown ce

SUBMITTER: Watterson WJ 

PROVIDER: S-EPMC7351490 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature