Assignment of the Number of Equivalent Reference Fluorophores to Dyed Microspheres.

ABSTRACT: A procedure will be described to assign to each dyed microsphere a number called the Equivalent number of Reference Fluorophores (ERF). The ERF unit gives the number of reference fluorophores in solution which produce the same fluorescence signal as a single dyed microsphere. In the first step, fluorescence measurements were carried out on serial dilutions of a solution of reference fluorophores. The resulting fluorescence intensities and the corresponding concentrations were used to calibrate the response of the fluorometer. The calibration consisted of establishing a linear relation between the intensities and concentrations. In the second step, the fluorescence intensity from a suspension of microspheres was measured in order to determine the equivalent concentration of reference fluoro

SUBMITTER: Wang L 

PROVIDER: S-EPMC7351568 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature