Structure of the DOCK2-ELMO1 complex provides insights into regulation of the auto-inhibited state.

ABSTRACT: DOCK (dedicator of cytokinesis) proteins are multidomain guanine nucleotide exchange factors (GEFs) for RHO GTPases that regulate intracellular actin dynamics. DOCK proteins share catalytic (DOCK^DHR2) and membrane-associated (DOCK^DHR1) domains. The structurally-related DOCK1 and DOCK2 GEFs are specific for RAC, and require ELMO (engulfment and cell motility) proteins for function. The N-terminal RAS-binding domain (RBD) of ELMO (ELMO^RBD) interacts with RHOG to modulate DOCK1/2 activity. Here, we determine the cryo-EM structures of DOCK2-ELMO1 alone, and as a ternary complex with RAC1, together with the crystal structure of a RHOG-ELMO2^RBD complex. The binary DOCK2-ELMO1 complex adopts a closed, auto-inhibited conformation. Relief of auto-inhibiti

SUBMITTER: Chang L 

PROVIDER: S-EPMC7351999 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature