Design and development of a chimeric vaccine candidate against zoonotic hepatitis E and foot-and-mouth disease.

ABSTRACT:

Background

Zoonotic hepatitis E virus (HEV) infection emerged as a serious threat in the industrialized countries. The aim of this study is exploring a new approach for the control of zoonotic HEV in its main host (swine) through the design and development of an economically interesting chimeric vaccine against HEV and against a devastating swine infection: the foot-and-mouth disease virus (FMDV) infection.

Results

First, we adopted a computational approach for rational and effective screening of the different HEV-FMDV chimeric proteins. Next, we further expressed and purified the selected chimeric immunogens in Escherichia coli (E. coli) using molecular cloning techniques. Finally, we assessed the antigenicity and immunogenicity profiles of the chimeric vaccine candidates.

SUBMITTER: Behloul N 

PROVIDER: S-EPMC7352093 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature