Involvement of POLA2 in Double Strand Break Repair and Genotoxic Stress.

ABSTRACT: Cellular survival is dependent on the efficient replication and transmission of genomic information. DNA damage can be introduced into the genome by several different methods, one being the act of DNA replication. Replication is a potent source of DNA damage and genomic instability, especially through the formation of DNA double strand breaks (DSBs). DNA polymerase alpha is responsible for replication initiation. One subunit of the DNA polymerase alpha replication machinery is POLA2. Given the connection between replication and genomic instability, we decided to examine the role of POLA2 in DSB repair, as little is known about this topic. We found that loss of POLA2 leads to an increase in spontaneous DSB formation. Loss of POLA2 also slows DSB repair kinetics after treatment with etoposid

SUBMITTER: Dang TT 

PROVIDER: S-EPMC7352189 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature