Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7.

ABSTRACT: Proteomics characterization of KAIMRC1 cell line, a naturally immortalized breast cancer cells, is described in comparison to MCF-7 and MDA-MB-231 breast cancer cells. Quantitative proteomics analysis using the tandem mass tag (TMT)-labeled technique in conjunction with the phosphopeptide enrichment method was used to perform comparative profiling of proteins and phosphoproteins in the three cell lines. In total, 673 proteins and 33 Phosphoproteins were differentially expressed among these cell lines. These proteins are involved in several key cellular pathways that include DNA replication and repair, splicing machinery, amino acid metabolism, cellular energy, and estrogen signaling pathway. Many of the differentially expressed proteins are associated with different types of tumors includi

SUBMITTER: Alghanem B 

PROVIDER: S-EPMC7352455 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature