Tracing Nutrient Flux Following Monocarboxylate Transporter-1 Inhibition with AZD3965.

ABSTRACT: The monocarboxylate transporter 1 (MCT1) is a key element in tumor cell metabolism and inhibition of MCT1 with AZD3965 is undergoing clinical trials. We aimed to investigate nutrient fluxes associated with MCT1 inhibition by AZD3965 to identify possible biomarkers of drug action. We synthesized an ¹⁸F-labeled lactate analogue, [¹⁸F]-S-fluorolactate ([¹⁸F]-S-FL), that was used alongside [¹⁸F]fluorodeoxyglucose ([¹⁸F]FDG), and ¹³C-labeled glucose and lactate, to investigate the modulation of metabolism with AZD3965 in diffuse large B-cell lymphoma models in NOD/SCID mice. Comparative analysis of glucose and lactate-based probes showed a preference for glycolytic metabolism in vitro, whereas in vivo, both glucose

SUBMITTER: Braga M 

PROVIDER: S-EPMC7352845 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature