The splicing-factor Prp40 affects dynein-dynactin function in Aspergillus nidulans.

ABSTRACT: The multi-component cytoplasmic dynein transports cellular cargoes with the help of another multi-component complex dynactin, but we do not know enough about factors that may affect the assembly and functions of these proteins. From a genetic screen for mutations affecting early-endosome distribution in Aspergillus nidulans, we identified the prp40A^L438* mutation in Prp40A, a homologue of Prp40, an essential RNA-splicing factor in the budding yeast. Prp40A is not essential for splicing, although it associates with the nuclear splicing machinery. The prp40A^L438* mutant is much healthier than the ∆prp40A mutant, but both mutants exhibit similar defects in dynein-mediated early-endosome transport and nuclear distribution. In the prp40A<

SUBMITTER: Qiu R 

PROVIDER: S-EPMC7353152 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature