A Novel Inducible Prophage from <i>Burkholderia Vietnamiensis</i> G4 is Widely Distributed across the Species and Has Lytic Activity against Pathogenic <i>Burkholderia</i>.

ABSTRACT: Burkholderia species have environmental, industrial and medical significance, and are important opportunistic pathogens in individuals with cystic fibrosis (CF). Using a combination of existing and newly determined genome sequences, this study investigated prophage carriage across the species B. vietnamiensis, and also isolated spontaneously inducible prophages from a reference strain, G4. Eighty-one B. vietnamiensis genomes were bioinformatically screened for prophages using PHASTER (Phage Search Tool Enhanced Release) and prophage regions were found to comprise up to 3.4% of total genetic material. Overall, 115 intact prophages were identified and there was evidence of polylysogeny in 32 strains. A novel, inducible Mu-like phage (vB_BvM-G4P1) was isolated from B.

SUBMITTER: Weiser R 

PROVIDER: S-EPMC7354579 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature