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Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands.


ABSTRACT: Edible Llayta are cyanobacterial colonies consumed in the Andes highlands. Llayta and four isolated cyanobacteria strains were tested for cyanotoxins (microcystin, nodularin, cylindrospermopsin, saxitoxin and β-N-methylamino-L-alanine-BMAA) using molecular and chemical methods. All isolates were free of target genes involved in toxin biosynthesis. Only DNA from Llayta amplified the mcyE gene. Presence of microcystin-LR and BMAA in Llayta extracts was discarded by LC/MS analyses. The analysed Llayta colonies have an incomplete microcystin biosynthetic pathway and are a safe food ingredient.

SUBMITTER: Galetovic A 

PROVIDER: S-EPMC7354591 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands.

Galetović Alexandra A   Azevedo Joana J   Castelo-Branco Raquel R   Oliveira Flavio F   Gómez-Silva Benito B   Vasconcelos Vitor V  

Toxins 20200609 6


Edible Llayta are cyanobacterial colonies consumed in the Andes highlands. Llayta and four isolated cyanobacteria strains were tested for cyanotoxins (microcystin, nodularin, cylindrospermopsin, saxitoxin and β-N-methylamino-L-alanine-BMAA) using molecular and chemical methods. All isolates were free of target genes involved in toxin biosynthesis. Only DNA from Llayta amplified the <i>mcy</i>E gene. Presence of microcystin-LR and BMAA in Llayta extracts was discarded by LC/MS analyses. The analy  ...[more]

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