Potential of Using Cell-Free DNA and miRNA in Breast Milk to Screen Early Breast Cancer.

ABSTRACT:

Objective

An ideal sample source is critical for more reliable and sensitive early detection of nucleic acid changes associated with breast cancer. Breast milk (BM) is a good noninvasive origin for genetic testing of early breast cancer, but cells in BM are easily disintegrated. So we investigate here whether cell-free nucleic acid (cfNA) exists in BM in a more stable form and whether the quality of BM cfNA is good enough for genetic testing.

Methods

A self-designed qRT-PCR method was used to measure the existence and abundance of cfDNA. Quality of cfDNA and cfRNA were detected by capillary electrophoresis. Whole genome bisulfite sequencing and miRNA sequencing were used to explore the sources of cfDNA and cell-free miRNA in BM. The copy number analysis and z-test bas

SUBMITTER: Song Q 

PROVIDER: S-EPMC7354639 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature