Apolipoprotein E expression pattern in human induced pluripotent stem cells during in vitro neural induction.

ABSTRACT: Apolipoprotein E (APOE) is a multifunctional protein that plays significant roles in important cellular mechanisms in peripheral tissues and is as well expressed in the central nervous system, notably by adult neural stem cells (NSCs) in the hippocampus. Evidence from animal studies suggest that APOE is critical for adult NSC maintenance. However, whether APOE has the potential to play a similar role in human NSCs has not been directly investigated. To address this question, we conducted a focused study characterising APOE gene and protein expression in an in vitro model of neural differentiation utilising human induced pluripotent stem cells. We found that APOE gene expression was dramatically decreased as the cells became more differentiated, indicating that APOE<

SUBMITTER: Lee H 

PROVIDER: S-EPMC7355222 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature