Light Regulation of Two New Manganese Peroxidase-Encoding Genes in Trametes polyzona KU-RNW027.

ABSTRACT: To better understand the light regulation of ligninolytic systems in Trametes polyzona KU-RNW027, ligninolytic enzymes-encoding genes were identified and analyzed to determine their transcriptional regulatory elements. Elements of light regulation were investigated in submerged culture. Three ligninolytic enzyme-encoding genes, mnp1, mnp2, and lac1, were found. Cloning of the genes encoding MnP1 and MnP2 revealed distinct deduced amino acid sequences with 90% and 86% similarity to MnPs in Lenzites gibbosa, respectively. These were classified as new members of short-type hybrid MnPs in subfamily A.2 class II fungal secretion heme peroxidase. A light responsive element (LRE), composed of a 5'-CCRCCC-3' motif in both mnp promoters, is reported. Light

SUBMITTER: Lueangjaroenkit P 

PROVIDER: S-EPMC7355636 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature