Microscale Thermophoresis as a Screening Tool to Predict Melanin Binding of Drugs.

ABSTRACT: Interactions between drugs and melanin pigment may have major impacts on pharmacokinetics. Therefore, melanin binding can modify the efficacy and toxicity of medications in ophthalmic and other disease of pigmented tissues, such as melanoma. As melanin is present in many pigmented tissues in the human body, investigation of pigment binding is relevant in drug discovery and development. Conventionally, melanin binding assays have been performed using an equilibrium binding study followed by chemical analytics, such as LC/MS. This approach is laborious, relatively slow, and limited to facilities with high performance quantitation instrumentation. We present here a screening of melanin binding with label-free microscale thermophoresis (MST) that utilizes the natural autofluorescence of melani

SUBMITTER: Hellinen L 

PROVIDER: S-EPMC7355663 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature