AP-TSS: A New Method for the Analysis of RNA Expression from Particular and Challenging Transcription Start Sites.

ABSTRACT: Alternative promoter usage involved in the regulation of transcription, splicing, and translation contributes to proteome diversity and is involved in a large number of diseases, in particular, cancer. Epigenetic mechanisms and cis regulatory elements are involved in alternative promoter activity. Multiple transcript isoforms can be produced from a gene, due to the initiation of transcription at different transcription start sites (TSS). These transcripts may not have regions that allow discrimination during RT-qPCR, making quantification technically challenging. This study presents a general method for the relative quantification of a transcript synthesized from a particular TSS that we called AP-TSS (analysis of particular TSS). AP-TSS is based on the specific elongation of the cDNA of i

SUBMITTER: Le Berre G 

PROVIDER: S-EPMC7355800 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature