Continuous Cell Lines from the European Biting Midge Culicoides nubeculosus (Meigen, 1830).

ABSTRACT: Culicoides biting midges (Diptera: Ceratopogonidae) transmit arboviruses of veterinary or medical importance, including bluetongue virus (BTV) and Schmallenberg virus, as well as causing severe irritation to livestock and humans. Arthropod cell lines are essential laboratory research tools for the isolation and propagation of vector-borne pathogens and the investigation of host-vector-pathogen interactions. Here we report the establishment of two continuous cell lines, CNE/LULS44 and CNE/LULS47, from embryos of Culicoides nubeculosus, a midge distributed throughout the Western Palearctic region. Species origin of the cultured cells was confirmed by polymerase chain reaction (PCR) amplification and sequencing of a fragment of the cytochrome oxidase1 gene, and the absence of bacterial contam

SUBMITTER: Bell-Sakyi L 

PROVIDER: S-EPMC7356041 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature