Detecting Cryptosporidium in Stool Samples Submitted to a Reference Laboratory.

ABSTRACT: When considering methods of detecting Cryptosporidium in patient samples, clinical and public health laboratories have historically relied primarily on microscopy. However, microscopy is time intensive and requires trained personnel to accurately identify pathogens that are present. Even with skilled analysts, the parasitemia level has the potential to fall below the level of detection. In addition, public health laboratories do not always receive specimens in fixatives that are compatible with the desired microscopic method. Antigen-based and molecular methods have proven to be effective at identifying Cryptosporidium at low levels and require less training and hands-on time. Here, we have developed and validated a real-time polymerase chain reaction (RT-PCR) laboratory-deve

SUBMITTER: Mergen K 

PROVIDER: S-EPMC7356455 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature