The Power of Three in Cannabis Shotgun Proteomics: Proteases, Databases and Search Engines.

ABSTRACT: Cannabis research has taken off since the relaxation of legislation, yet proteomics is still lagging. In 2019, we published three proteomics methods aimed at optimizing protein extraction, protein digestion for bottom-up and middle-down proteomics, as well as the analysis of intact proteins for top-down proteomics. The database of Cannabis sativa proteins used in these studies was retrieved from UniProt, the reference repositories for proteins, which is incomplete and therefore underrepresents the genetic diversity of this non-model species. In this fourth study, we remedy this shortcoming by searching larger databases from various sources. We also compare two search engines, the oldest, SEQUEST, and the most popular, Mascot. This shotgun proteomics experiment also utilizes the powe

SUBMITTER: Vincent D 

PROVIDER: S-EPMC7356525 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature