In Vitro Propagation of an Endangered Helianthus Verticillatus by Axillary Bud Proliferation.

ABSTRACT: Helianthus verticillatus (Asteraceae), whorled sunflower, is a perennial species restricted to a few locations in the Southeastern United States. Habitat loss has caused H. verticillatus to become rare, and since 2014, it has been federally listed as an endangered species. As a part of the recovery plan for the restoration and protection of H. verticillatus, an efficient micropropagation protocol based on axillary shoot proliferation was developed. Various concentrations of 6-benzylaminopurine (BAP; 0 to 4.44 µM) were examined for their morphogenetic potential in the regeneration of six genotypes of H. verticillatus from the nodal explants derived from greenhouse-grown plants. Both the BAP concentration and genotype had significant effects on the regeneration capacity of H. verticillatus.

SUBMITTER: Nowakowska M 

PROVIDER: S-EPMC7356533 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature