Genotypic and Phenotypic Resistance to Clarithromycin in Helicobacter pylori Strains.

ABSTRACT:

Background

The increasing prevalence of antimicrobial resistance, together with the lack of novel treatment options, negatively affects successful eradication of Helicobacter pylori. The aim of this study was to investigate genetic mutations in the 23S rRNA genes, which is associated with clarithromycin resistance, and to determine the clinical impact of genotype on phenotypic antimicrobial resistance.

Methods

A total of 46 H. pylori strains were obtained from 13 patients, before and after unsuccessful eradication with clarithromycin-based triple therapy. The phenotypic resistance of each H. pylori strain was determined by minimum inhibitory concentration against clarithromycin using the serial two-fold agar dilution method. The genomic sequences of 23S

SUBMITTER: Gong EJ 

PROVIDER: S-EPMC7356929 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature