EGFP Reporters for Direct and Sensitive Detection of Mutagenic Bypass of DNA Lesions.

ABSTRACT: The sustainment of replication and transcription of damaged DNA is essential for cell survival under genotoxic stress; however, the damage tolerance of these key cellular functions comes at the expense of fidelity. Thus, translesion DNA synthesis (TLS) over damaged nucleotides is a major source of point mutations found in cancers; whereas erroneous bypass of damage by RNA polymerases may contribute to cancer and other diseases by driving accumulation of proteins with aberrant structure and function in a process termed "transcriptional mutagenesis" (TM). Here, we aimed at the generation of reporters suited for direct detection of miscoding capacities of defined types of DNA modifications during translesion DNA or RNA synthesis in human cells. We performed a systematic phenotypic screen of 2

SUBMITTER: Rodriguez-Alvarez M 

PROVIDER: S-EPMC7357151 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature