Identifying and Characterizing Stress Pathways of Concern for Consumer Safety in Next-Generation Risk Assessment.

ABSTRACT: Many substances for which consumer safety risk assessments need to be conducted are not associated with specific toxicity modes of action, but rather exhibit nonspecific toxicity leading to cell stress. In this work, a cellular stress panel is described, consisting of 36 biomarkers representing mitochondrial toxicity, cell stress, and cell health, measured predominantly using high content imaging. To evaluate the panel, data were generated for 13 substances at exposures consistent with typical use-case scenarios. These included some that have been shown to cause adverse effects in a proportion of exposed humans and have a toxicological mode-of-action associated with cellular stress (eg, doxorubicin, troglitazone, and diclofenac), and some that are not associated with adverse effects due to

SUBMITTER: Hatherell S 

PROVIDER: S-EPMC7357173 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature