Ethanol Metabolism Dynamics in Clostridium ljungdahlii Grown on Carbon Monoxide.

ABSTRACT: Bioethanol production from syngas using acetogenic bacteria has attracted considerable attention in recent years. However, low ethanol yield is the biggest challenge that prevents the commercialization of syngas fermentation into biofuels using microbial catalysts. The present study demonstrated that ethanol metabolism plays an important role in recycling NADH/NAD⁺ during autotrophic growth. Deletion of bifunctional aldehyde/alcohol dehydrogenase (adhE) genes leads to significant growth deficiencies in gas fermentation. Using specific fermentation technology in which the gas pressure and pH were constantly controlled at 0.1 MPa and 6.0, respectively, we revealed that ethanol was formed during the exponential phase, closely accompanied by biomass production. Then, ethanol

SUBMITTER: Liu ZY 

PROVIDER: S-EPMC7357473 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature