Xylose-Inducible Promoter Tools for Pseudomonas Species and Their Use in Implicating a Role for the Type II Secretion System Protein XcpQ in the Inhibition of Corneal Epithelial Wound Closure.

ABSTRACT: Tunable control of gene expression is an invaluable tool for biological experiments. In this study, we describe a new xylose-inducible promoter system and evaluate it in both Pseudomonas aeruginosa and Pseudomonas fluorescens The P_xut promoter, derived from the P. fluorescensxut operon, was incorporated into a broad-host-range pBBR1-based plasmid and was compared to the Escherichia coli-derived P_BAD promoter using gfp as a reporter. Green fluorescent protein (GFP) fluorescence from the P_xut promoter was inducible in both Pseudomonas species, but not in E. coli, which may facilitate the cloning of genes toxic to E. coli to generate plasmids. The P_xut promote

SUBMITTER: Callaghan JD 

PROVIDER: S-EPMC7357479 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature