Encapsulated Nanodroplet Crystallization of Organic-Soluble Small Molecules.

ABSTRACT: Single-crystal X-ray diffraction analysis (SCXRD) constitutes a universal approach for the elucidation of molecular structure and the study of crystalline forms. However, the discovery of viable crystallization conditions remains both experimentally challenging and resource intensive in both time and the quantity of analyte(s). We report a robot-assisted, high-throughput method for the crystallization of organic-soluble small molecules in which we employ only micrograms of analyte per experiment. This allows hundreds of crystallization conditions to be screened in parallel with minimal overall sample requirements. Crystals suitable for SCXRD are grown from nanoliter droplets of a solution of analyte in organic solvent(s), each of which is encapsulated within an inert oil to control the rat

SUBMITTER: Tyler AR 

PROVIDER: S-EPMC7357602 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature