MRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage.

ABSTRACT: Caenorhabditis elegans early embryos generate cell-specific transcriptomes despite lacking active transcription, thereby presenting an opportunity to study mechanisms of post-transcriptional regulatory control. We observed that some cell-specific mRNAs accumulate non-homogenously within cells, localizing to membranes, P granules (associated with progenitor germ cells in the P lineage) and P-bodies (associated with RNA processing). The subcellular distribution of transcripts differed in their dependence on 3'UTRs and RNA binding proteins, suggesting diverse regulatory mechanisms. Notably, we found strong but imperfect correlations between low translational status and P granule localization within the progenitor germ lineage. By uncoupling translation from mRNA localization, we untang

SUBMITTER: Parker DM 

PROVIDER: S-EPMC7358130 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature