Validation of Droplet Digital Polymerase Chain Reaction for Salmonella spp. Quantification.

ABSTRACT: Salmonellosis is a foodborne disease caused by Salmonella spp. Although cell culture is the gold standard for its identification, validated molecular methods are becoming an alternative, because of their rapidity, selectivity, and specificity. A simplex and duplex droplet digital polymerase chain reaction (ddPCR)-based method for the identification and quantification of Salmonella using ttr, invA, hilA, spaQ, and siiA gene sequences was validated. The method has high specificity, working interval between 8 and 8,000 cp/?L in ddPCR reaction, a limit of detection of 0.5 copies/?L, and precision ranging between 5 and 10% measured as a repeatability standard deviation. The relative standard measurement uncertainty was between 2 and 12%. This tool will improve food safety in national consumption products and will increase the competitiveness in agricultural product trade.

SUBMITTER: Villamil C 

PROVIDER: S-EPMC7358645 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature