The circular RNA landscape in specific peripheral blood mononuclear cells of critically ill patients with sepsis.

ABSTRACT:

Background

Dysregulation of the host immune response is a pathognomonic feature of sepsis. Abnormal physiological conditions are understood to shift efficient linear splicing of protein-coding RNA towards non-canonical splicing, characterized by the accumulation of non-coding circularized (circ)RNA. CircRNAs remain unexplored in specific peripheral blood mononuclear cells (PBMCs) during sepsis. We here sought to identify and characterize circRNA expression in specific PBMCs of patients with sepsis due to community-acquired pneumonia (CAP) relative to healthy subjects.

Methods

The study comprised a discovery cohort of six critically ill patients diagnosed with sepsis due to community-acquired pneumonia and four (age, gender matched) healthy subjects. PBMCs were isolated, and

SUBMITTER: Khan HN 

PROVIDER: S-EPMC7359566 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature